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Alexey Egorov

Picture of Alexey Egorov

Date of Birth: 02/19/1943

Age: 73

Place of birth: Moscow

Citizenship: Russia

Background

After graduating from high school in Moscow in 1960 AM Egorov joined the Department of Biophysics, Faculty of Moscow State University named after MV Biologopochvennogo Lomonosov. After graduation, he began working in the name of LM interdepartmental Laboratory of Bioorganic Chemistry White Lake, where in 1971 he defended his thesis on the quaternary structure of the human myeloma immunoglobulin. In the following years he worked at the Institute of Chemical Physics, Academy of Sciences of the USSR, where he continued to study the structure of proteins and began development of highly sensitive methods of immunoassay leukemias of humans and animals.

Since 1973, after the transition to the Department of Chemistry, Moscow State University Lomonosov AM Egorov organized problem laboratory SCS & T engineering enzymology, led up to the present time. The laboratory began to work on the creation of biocatalysts and their use in various fields of science and industry. On the basis of the laboratory in the future organized by the Department of enzymology under the leadership of Corresponding Member of the USSR Academy IV Berezina.

Main research interests AM Egorov focused on studying the mechanism of action of enzymes involved in the oxidation of compounds C-1, NAD-dependent dehydrogenases methanol, formaldehyde, and formate dehydrogenase and NAD-dependent hydrogenase. The complex of these enzymes to create a highly efficient system of producing hydrogen for fuel cells. The combination of electrochemical and enzymological research led to the development of a new direction - biosensors, which flow bioelectrode for glucose determination were created.

Another enzyme, which is studied in detail in the lab AM Egorov was horseradish peroxidase, which is one of the most common tokens in ELISA. Launched in 1973 under the direction of AM Egorova research on enzyme immunoassay are widely used in the country for the first infectious, and later for noncommunicable diseases. An integrated approach for enzyme immunoassay designed to detect both proteins and haptens. Thus, we created a comprehensive system diagnostics thyroid hormones and proteins, steroid hormones, cancer antigens.

Given that the reaction catalyzed by horseradish peroxidase, associated with the formation of free radicals, led by AM Egorov reaction studied and developed by enhanced chemiluminescence detection biosensors for chemiluminescent reactions in various formats, including the membrane surfaces, in the flow mode in plastic plates.

Further investigation AM Yegorova were aimed at the development of methods for producing genetically engineered peroxidase, expressed in E. coli. It was shown that the deglycosylated peroxidase has unusual properties of direct electron transfer when immobilized on a gold electrode. This trend opens up the possibility of creating high-sensitivity biosensors new generation for a variety of compounds.

Under the leadership of AM Egorova developed methods for peroxidase conjugates with various proteins by genetic engineering techniques. This approach opens the possibility of creating new technology for the preparation of conjugates of an enzyme immunoassay, new drug compounds with anti-cancer and anti-bacterial antigens and properties.

To study the structure of the immunoglobulin under the direction of AM Egorov studied to obtain genetically engineered antibodies and their conjugates to antigens, which allows to standardize and enhance the sensitivity of enzyme immunoassay. Immunochemistry fundamental problem remains disclosure mechanism specific interaction of antibodies with haptens, antigens of low molecular weight that forms a cross-reaction with many closely related compounds.

Systematic studies of the effect of an immunogen complex structure, the spacer and the labeled hapten immunization conditions allow for example a wide range of compounds (antibiotics, drugs, pesticides and other chemicals) to construct the theory of group and individual specificity of the immunoassay. An important contribution to the development of methods for the determination of haptens is the development of methods of polarization immunoassay.

In 1998-2002 AM Egorov worked as General Director of the State Scientific Center of antibiotics (GNTSA), where under his revived research in the search for new antibiotics, immunomodulators study, mechanisms of Resistance of microorganisms to antibiotics, restored industrial biotech production base asparaginase and genetically engineered insulin. Much attention was paid to the creation of new methods of analysis and control processes.

Under the leadership of AM Yegorova in GNTSA restored the work of the Scientific Board of HAC, edition of the journal